Detecting Proteins at 280 nm by UV-Vis: Why? How?

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Explains protein detection at 280 nm via UV-Vis, covering aromatic amino acid absorption physics and practical method selection. Both why and how of a standard technique.

A 12-minute video from Catalyst University: “Detecting Proteins at 280 nm by UV-Vis: Why? How?”.

Video by Catalyst University, played through YouTube's embedded player.

Part of UV-Vis Spectroscopy, Biochemical Laboratory Techniques, Protein/Enzyme Function